Characterization of stanniocalcin-1 expression in macrophage differentiation

Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- anti-inflammatory functions differentiating macrophage, however, not clear. In this study, our data showed that phorbol 12-myristate 13-acetate (PMA) treatment induced human leukemia monocytic cells (ThP-1) differentiation to M0 macrophages. was accompanied by significant increase mRNA expression levels STC1, pro-inflammatory cytokine TNFα, markers, CD163 & CD206. An intermitted removal PMA reduced TNFα but had no noticeable effects on markers. correlation markers macrophages investigated, using siRNASTC1-transfected PMA-induced cells. Consistently, transcripts IL-6 were significantly reduced. Moreover, LPS/IFNγ-induced M1-polarization remarkably higher than IL-4/IL-13-induced M2-macrophages M0-macrophages. Transcriptomic analysis M1-polarized revealed an upregulation TBC1 domain family member 3 (TBC1D3G). gene regulates payload macrophage-released extracellular vesicles mediate inflammation. conditioned media from found reduce Hep3B cell motility. suggest macrophage differentiation, preferentially M1 polarization.